(kIP3deg * (IP3_Cyt - IP3_basal))
0.16
0.16
Rate_IP3deg
100.0
(kf_IP3PH * KdIP3PH)
2.0
kf_IP3PH
kr_IP3PH
(((kf_IP3PH * IP3_Cyt) * PH_GFP_Cyt) - (kr_IP3PH * IP3_PHGFP_Cyt))
200.0
(kf_PIP2PH * KdPIP2PH)
2.0
kf_PIP2PH
kr_PIP2PH
(((kf_PIP2PH * PH_GFP_Cyt) * PIP2_PM) - (kr_PIP2PH * PIP2_PHGFP_PM))
((Rate_PIP2Synbasal + Rate_PIP2SynStim) * PIP_PM)
((kBasalSynPIP2 * 0.581 * ( - 1.0 + exp(((PIP2_basal - PIP2_PM) / PIP2_basal)))) * (PIP2_PM < PIP2_basal))
(kStimSynPIP2 * exp( - ((t - tauPIP2syn) / PIP2syndecay)) * (t > tauPIP2syn))
0.045
1072.0
0.01
0.05
10.0
Rate_PIP2Syn
((Ratebasal_PIPsyn + Ratestim_PIPsyn) * PI_PM)
((kBasalSynPIP * 0.581 * ( - 1.0 + exp(((PIP_basal - PIP_PM) / PIP_basal)))) * (PIP_PM < PIP_basal))
(kStimSynPIP * exp( - ((t - tauPIPsyn) / PIPsyndecay)) * (t > tauPIPsyn))
0.0015
2857.0
0.01
0.05
10.0
Rate_PIPSyn
(k_PIP2hyd * (PIP2_PM * PLC_act_PM))
1.188
Rate_PIP2_hyd
(intensity * IP3X_Cytosol * hv_Cytosol)
100000.0
J_IP3_uncaging
((KfPLCact * (PLC_PM * stim_PM * signal)) - (krPLCact * PLC_act_PM))
1000.0
((t > tau0) * exp( - ((t - tau0) / stimdecay)))
10.0
0.05
6.0
RatePLCact
(k_PIP2PHhyd * PLC_act_PM * PIP2_PHGFP_PM)
50.0
RatePIP2_PH_hyd
1.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.16
0.0
0.0
0.0
1200000.0
0.0
0.0
0.0
12000.0
0.0
0.0
0.0
2000.0
0.0
0.0
0.0
0.0
0.0
2.0
0.0015
0.0
0.16
100000.0
1000.0
0.0
96480.0
10.0
8314.0
0.0
2857.0
10.0
0.0
0.01
2000.0
1200000.0
1.188
1.0
0.263
0.16
1200000.0
0.16
0.0
12000.0
0.1
2.0
6.0
200.0
0.0
0.8
0.0
0.01
(1.0 / 602.0)
1.0
10.0
0.05
1.0
0.05
0.0
0.0
1072.0
300.0
100.0
0.045
12000.0
1000.0
0.16
50.0
0.05
(kf_PIP2PH * KdPIP2PH)
(((kf_PIP2PH * PH_GFP_Cyt) * PIP2_PM) - (kr_PIP2PH * PIP2_PHGFP_PM))
((t > tau0) * exp( - ((t - tau0) / stimdecay)))
(kStimSynPIP2 * exp( - ((t - tauPIP2syn) / PIP2syndecay)) * (t > tauPIP2syn))
((PLC_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PLC_act_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE))
((K_PLC_PM_total - (PLC_act_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE)) / (SurfToVol_PM * VolFract_Cytosol * KMOLE))
((KfPLCact * (PLC_PM * stim_PM * signal)) - (krPLCact * PLC_act_PM))
((kBasalSynPIP * 0.581 * ( - 1.0 + exp(((PIP_basal - PIP_PM) / PIP_basal)))) * (PIP_PM < PIP_basal))
(kStimSynPIP * exp( - ((t - tauPIPsyn) / PIPsyndecay)) * (t > tauPIPsyn))
((Ratebasal_PIPsyn + Ratestim_PIPsyn) * PI_PM)
(k_PIP2hyd * (PIP2_PM * PLC_act_PM))
(hv_Cytosol_init * (1.0 - VolFract_Nucleus) * VolFract_Cytosol)
(K_hv_Cytosol_total / ((1.0 - VolFract_Nucleus) * VolFract_Cytosol))
((kBasalSynPIP2 * 0.581 * ( - 1.0 + exp(((PIP2_basal - PIP2_PM) / PIP2_basal)))) * (PIP2_PM < PIP2_basal))
((Rate_PIP2Synbasal + Rate_PIP2SynStim) * PIP_PM)
SurfToVol_NM
(kf_IP3PH * KdIP3PH)
(((kf_IP3PH * IP3_Cyt) * PH_GFP_Cyt) - (kr_IP3PH * IP3_PHGFP_Cyt))
(SurfToVol_NM * VolFract_Nucleus / (1.0 - VolFract_Nucleus))
(SurfToVol_PM / (1.0 - VolFract_Nucleus))
0.0
(kIP3deg * (IP3_Cyt - IP3_basal))
(SurfToVol_PM * VolFract_Cytosol / (1.0 - VolFract_Cytosol))
(intensity * IP3X_Cytosol * hv_Cytosol)
(k_PIP2PHhyd * PLC_act_PM * PIP2_PHGFP_PM)
0.0
IP3_PHGFP_Cyt_init
- J_IP3_uncaging
IP3X_Cytosol_init
(J_PIP2_PH - RatePIP2_PH_hyd)
PIP2_PHGFP_PM_init
( - Rate_PIP2Syn + Rate_PIPSyn)
PIP_PM_init
(Rate_PIP2_hyd + RatePIP2_PH_hyd)
DAG_PM_init
RatePLCact
PLC_act_PM_init
J_IP3_PHGFP
4000.0
100000.0
100.0
300.0
0.16
0.16
2857.0
0.0
0.01
0.05
0.05
0.05
10.0
(1.0 / 602.0)
0.0015
0.8
0.0
142857.0
2.30769
0.0
0.0
96480.0
10.0
0.01
1.0
1.188
0.045
2000.0
0.0
142857.0
8314.0
1000.0
4000.0
100.0
0.0
0.1
6.0
4617.4
0.16
10.0
200.0
3.6923
0.0
2.30769
0.1
2.0
4000.0
50.0
0.16
0.263
Total PIP2 + both complexes = 12,000molec/um2
2.30769
0.0
3.6923
0.0
1.0
0.0
1.01446
0.0
0.16
0.0
4617.4
0.0
142857.0
0.0
1.0
0.0
4000.0
0.0
2857.0
0.0
2000.0
0.0
100.0
0.0
0.0
0.0
2.0
100.0
0.16
100000.0
1000.0
1.0
96480.0
8314.0
1.0
3.6923
2000.0
142857.0
2.30769
1.0
0.263
0.16
0.16
2857.0
4000.0
0.1
2.0
6.0
200.0
0.0
0.8
1.01446
(1.0 / 602.0)
10.0
0.05
1.0
1.0
4617.4
300.0
100.0
1000.0
50.0
(PI_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE)
( - (DAG_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) - (PIP2_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PH_GFP_Cyt_init * (1.0 - VolFract_Nucleus) * VolFract_Cytosol) + (IP3_PHGFP_Cyt_init * (1.0 - VolFract_Nucleus) * VolFract_Cytosol))
(((DAG_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PIP2_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + K_PH_GFP_Cyt_total - (IP3_PHGFP_Cyt * (1.0 - VolFract_Nucleus) * VolFract_Cytosol)) / ((1.0 - VolFract_Nucleus) * VolFract_Cytosol))
(kf_PIP2PH * KdPIP2PH)
((DAG_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PIP2_PHGFP_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PIP2_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE))
(( - (DAG_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + K_PIP2_PHGFP_PM_total - (PIP2_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE)) / (SurfToVol_PM * VolFract_Cytosol * KMOLE))
(((kf_PIP2PH * PH_GFP_Cyt) * PIP2_PM) - (kr_PIP2PH * PIP2_PHGFP_PM))
((t > tau0) * exp( - ((t - tau0) / stimdecay)))
((PLC_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PLC_act_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE))
((K_PLC_PM_total - (PLC_act_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE)) / (SurfToVol_PM * VolFract_Cytosol * KMOLE))
((KfPLCact * (PLC_PM * stim_PM * signal)) - (krPLCact * PLC_act_PM))
(PIP_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE)
(K_PIP_PM_total / (SurfToVol_PM * VolFract_Cytosol * KMOLE))
SurfToVol_NM
(K_PI_PM_total / (SurfToVol_PM * VolFract_Cytosol * KMOLE))
(kf_IP3PH * KdIP3PH)
(((kf_IP3PH * IP3_Cyt) * PH_GFP_Cyt) - (kr_IP3PH * IP3_PHGFP_Cyt))
(SurfToVol_NM * VolFract_Nucleus / (1.0 - VolFract_Nucleus))
(SurfToVol_PM / (1.0 - VolFract_Nucleus))
0.0
(kIP3deg * (IP3_Cyt - IP3_basal))
(SurfToVol_PM * VolFract_Cytosol / (1.0 - VolFract_Cytosol))
(intensity * IP3X_Cytosol * hv_Cytosol)
(k_PIP2PHhyd * PLC_act_PM * PIP2_PHGFP_PM)
J_IP3_PHGFP
IP3_PHGFP_Cyt_init
- J_IP3_uncaging
IP3X_Cytosol_init
((RatePIP2_PH_hyd * Kflux_PM_Cytosol * KMOLE) + J_IP3_uncaging - Rate_IP3deg - J_IP3_PHGFP)
IP3_Cyt_init
- J_PIP2_PH
PIP2_PM_init
RatePIP2_PH_hyd
DAG_PM_init
RatePLCact
PLC_act_PM_init
0.0
4617.4
100.0
1.0
4000.0
0.12
2857.0
1.0
2000.0
0.5
0.8
1.0
2.0
0.0
3.6923
0.0
0.16
0.1
0.0
6.0
0.16
0.08
100000.0
0.0
0.0
0.1
1000.0
(1.0 / 602.0)
142857.0
10.0
10.0
2.30769
300.0
96480.0
1.01446
8314.0
0.0
(4617.4 * 3.0)
100.0
1.0
4000.0
0.12
2857.0
1.0
2000.0
0.5
0.8
1.0
2.0
0.0
(3.6923 * 3.0)
0.0
0.16
0.1
0.0
6.0
0.16
0.08
100000.0
0.0
0.0
0.1
1000.0
(1.0 / 602.0)
142857.0
10.0
10.0
(2.30769 * 3.0)
300.0
96480.0
1.01446
8314.0
0.0
(4617.4 * 0.3)
100.0
1.0
4000.0
0.12
2857.0
1.0
2000.0
0.5
0.8
1.0
2.0
0.0
(3.6923 * 0.3)
0.0
0.16
0.1
0.0
6.0
0.16
0.08
100000.0
0.0
0.0
0.1
1000.0
(1.0 / 602.0)
142857.0
10.0
10.0
(2.30769 * 0.3)
300.0
96480.0
1.01446
8314.0
0.0
4617.4
100.0
1.0
4000.0
0.12
2857.0
1.0
2000.0
0.5
0.8
1.0
2.0
0.0
3.6923
0.0
0.16
0.1
0.0
6.0
0.16
0.16
100000.0
0.0
0.0
0.1
1000.0
(1.0 / 602.0)
142857.0
10.0
10.0
2.30769
300.0
96480.0
0.0
8314.0
(copied from Average Exp Params - Comp) (copied from SS for Exp params) (copied from steady state compartment)
5.46296
0.0
0.437037
0.0
0.0
0.0
0.0
0.0
0.16
0.0
2928.0
0.0
142857.0
0.0
1.0
0.0
1072.0
0.0
2857.0
0.0
2000.0
0.0
100.0
0.0
0.0
0.0
2.0
0.0015
100.0
0.16
1000.0
0.0
96480.0
10.0
8314.0
1.0
2857.0
10.0
0.437037
0.0
0.01
2000.0
142857.0
1.188
5.46296
0.0
0.263
0.16
0.16
2857.0
1072.0
0.1
2.0
6.0
200.0
0.0
0.8
0.0
0.01
(1.0 / 602.0)
10.0
0.05
1.0
0.05
1.0
2928.0
1072.0
300.0
100.0
0.045
1000.0
50.0
0.05
( - (DAG_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) - (PIP_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) - (PIP2_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) - (PI_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PH_GFP_Cyt_init * (1.0 - VolFract_Nucleus) * VolFract_Cytosol) + (IP3_PHGFP_Cyt_init * (1.0 - VolFract_Nucleus) * VolFract_Cytosol))
(((DAG_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PIP_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PIP2_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PI_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + K_PH_GFP_Cyt_total - (IP3_PHGFP_Cyt * (1.0 - VolFract_Nucleus) * VolFract_Cytosol)) / ((1.0 - VolFract_Nucleus) * VolFract_Cytosol))
(kf_PIP2PH * KdPIP2PH)
((DAG_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PIP_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PIP2_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PIP2_PHGFP_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PI_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE))
(( - (DAG_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) - (PIP_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) - (PIP2_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + K_PIP2_PHGFP_PM_total - (PI_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE)) / (SurfToVol_PM * VolFract_Cytosol * KMOLE))
(((kf_PIP2PH * PH_GFP_Cyt) * PIP2_PM) - (kr_PIP2PH * PIP2_PHGFP_PM))
((t > tau0) * exp( - ((t - tau0) / stimdecay)))
(kStimSynPIP2 * exp( - ((t - tauPIP2syn) / PIP2syndecay)) * (t > tauPIP2syn))
((PLC_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PLC_act_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE))
((K_PLC_PM_total - (PLC_act_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE)) / (SurfToVol_PM * VolFract_Cytosol * KMOLE))
((KfPLCact * (PLC_PM * stim_PM * signal)) - (krPLCact * PLC_act_PM))
((kBasalSynPIP * 0.581 * ( - 1.0 + exp(((PIP_basal - PIP_PM) / PIP_basal)))) * (PIP_PM < PIP_basal))
(kStimSynPIP * exp( - ((t - tauPIPsyn) / PIPsyndecay)) * (t > tauPIPsyn))
((Ratebasal_PIPsyn + Ratestim_PIPsyn) * PI_PM)
(k_PIP2hyd * (PIP2_PM * PLC_act_PM))
((kBasalSynPIP2 * 0.581 * ( - 1.0 + exp(((PIP2_basal - PIP2_PM) / PIP2_basal)))) * (PIP2_PM < PIP2_basal))
((Rate_PIP2Synbasal + Rate_PIP2SynStim) * PIP_PM)
SurfToVol_NM
(kf_IP3PH * KdIP3PH)
(((kf_IP3PH * IP3_Cyt) * PH_GFP_Cyt) - (kr_IP3PH * IP3_PHGFP_Cyt))
(SurfToVol_NM * VolFract_Nucleus / (1.0 - VolFract_Nucleus))
(SurfToVol_PM / (1.0 - VolFract_Nucleus))
0.0
(kIP3deg * (IP3_Cyt - IP3_basal))
(SurfToVol_PM * VolFract_Cytosol / (1.0 - VolFract_Cytosol))
(k_PIP2PHhyd * PLC_act_PM * PIP2_PHGFP_PM)
J_IP3_PHGFP
IP3_PHGFP_Cyt_init
((RatePIP2_PH_hyd * Kflux_PM_Cytosol * KMOLE) + (Rate_PIP2_hyd * Kflux_PM_Cytosol * KMOLE) - Rate_IP3deg - J_IP3_PHGFP)
IP3_Cyt_init
- Rate_PIPSyn
PI_PM_init
( - Rate_PIP2_hyd - J_PIP2_PH + Rate_PIP2Syn)
PIP2_PM_init
( - Rate_PIP2Syn + Rate_PIPSyn)
PIP_PM_init
(Rate_PIP2_hyd + RatePIP2_PH_hyd)
DAG_PM_init
RatePLCact
PLC_act_PM_init
2.0
1.0
0.16
2.0
0.8
0.0
300.0
0.1
0.05
0.048
0.1
5.0E-4
2000.0
0.0
142857.0
0.0
0.92
4000.0
0.0
1000.0
1.0
0.0
1.0
0.0055
0.5
2857.0
0.0
1.0
0.12
0.0
0.16
0.0
8314.0
0.0
0.05
10.0
0.019
2857.0
4000.0
(1.0 / 602.0)
0.08
1.0
0.05
2.4
100.0
96480.0
1.0
S:V=.5 optimized rates
2.0
1.0
0.16
0.1
0.8
3.6923
300.0
0.1
0.05
0.048
0.1
5.0E-4
2000.0
0.0
142857.0
0.0
0.92
4000.0
0.0
1000.0
1.0
2.30769
1.0
0.0055
0.5
2857.0
4617.4
1.0
0.12
0.0
0.16
0.0
8314.0
0.0
0.05
10.0
0.019
2857.0
4000.0
(1.0 / 602.0)
0.08
1.0
0.05
2.4
100.0
96480.0
1.0
S:V=.5 optimized rates
2.0
1.0
0.16
0.1
0.8
(3.6923 * 3.0)
300.0
0.1
0.05
0.048
0.1
5.0E-4
2000.0
0.0
142857.0
0.0
0.92
4000.0
0.0
1000.0
1.0
(2.30769 * 3.0)
1.0
0.0055
0.5
2857.0
(4617.4 * 3.0)
1.0
0.12
0.0
0.16
0.0
8314.0
0.0
0.05
10.0
0.019
2857.0
4000.0
(1.0 / 602.0)
0.08
1.0
0.05
2.4
100.0
96480.0
1.0
S:V=.5 optimized rates
2.0
1.0
0.16
0.1
0.8
(3.6923 * 0.3)
300.0
0.1
0.05
0.048
0.1
5.0E-4
2000.0
0.0
142857.0
0.0
0.92
4000.0
0.0
1000.0
1.0
(2.30769 * 0.3)
1.0
0.0055
0.5
2857.0
(4617.4 * 0.3)
1.0
0.12
0.0
0.16
0.0
8314.0
0.0
0.05
10.0
0.019
2857.0
4000.0
(1.0 / 602.0)
0.08
1.0
0.05
2.4
100.0
96480.0
1.0
S:V=.5 optimized rates
2.0
1.0
0.16
0.1
0.8
3.6923
300.0
0.1
0.05
0.048
0.1
5.0E-4
2000.0
0.0
142857.0
0.0
0.0
4000.0
0.0
1000.0
1.0
2.30769
1.0
0.0055
0.5
2857.0
4617.4
1.0
0.12
0.0
0.16
0.0
8314.0
0.0
0.05
10.0
0.0
2857.0
4000.0
(1.0 / 602.0)
0.08
1.0
0.05
2.4
100.0
96480.0
1.0
S:V=.5 optimized rates
(copied from uncaging) (copied from Average Exp Params - Comp) (copied from SS for Exp params) (copied from steady state compartment)
2.30769
0.0
3.6923
0.0
0.0
0.0
0.0
0.0
0.16
0.0
4617.4
0.0
142857.0
0.0
1.0
0.0
4000.0
0.0
2857.0
0.0
2000.0
0.0
100.0
0.0
0.0
0.0
2.0
0.0015
100.0
0.16
1000.0
0.0
96480.0
10.0
8314.0
1.0
2857.0
10.0
3.6923
0.0
0.01
2000.0
142857.0
1.188
2.30769
0.0
0.5
0.16
0.16
2857.0
4000.0
0.1
2.0
6.0
200.0
0.0
0.8
0.0
0.01
(1.0 / 602.0)
10.0
0.05
1.0
0.05
1.0
4617.4
1072.0
300.0
100.0
0.045
1000.0
0.16
50.0
0.05
( - (DAG_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) - (PIP_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) - (PIP2_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) - (PI_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PH_GFP_Cyt_init * (1.0 - VolFract_Nucleus) * VolFract_Cytosol) + (IP3_PHGFP_Cyt_init * (1.0 - VolFract_Nucleus) * VolFract_Cytosol))
(((DAG_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PIP_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PIP2_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PI_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + K_PH_GFP_Cyt_total - (IP3_PHGFP_Cyt * (1.0 - VolFract_Nucleus) * VolFract_Cytosol)) / ((1.0 - VolFract_Nucleus) * VolFract_Cytosol))
(kf_PIP2PH * KdPIP2PH)
((DAG_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PIP_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PIP2_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PIP2_PHGFP_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PI_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE))
(( - (DAG_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) - (PIP_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) - (PIP2_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + K_PIP2_PHGFP_PM_total - (PI_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE)) / (SurfToVol_PM * VolFract_Cytosol * KMOLE))
(((kf_PIP2PH * PH_GFP_Cyt) * PIP2_PM) - (kr_PIP2PH * PIP2_PHGFP_PM))
((t > tau0) * exp( - ((t - tau0) / stimdecay)))
(kStimSynPIP2 * exp( - ((t - tauPIP2syn) / PIP2syndecay)) * (t > tauPIP2syn))
((PLC_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PLC_act_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE))
((K_PLC_PM_total - (PLC_act_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE)) / (SurfToVol_PM * VolFract_Cytosol * KMOLE))
((KfPLCact * (PLC_PM * stim_PM * signal)) - (krPLCact * PLC_act_PM))
((kBasalSynPIP * 0.581 * ( - 1.0 + exp(((PIP_basal - PIP_PM) / PIP_basal)))) * (PIP_PM < PIP_basal))
(kStimSynPIP * exp( - ((t - tauPIPsyn) / PIPsyndecay)) * (t > tauPIPsyn))
((Ratebasal_PIPsyn + Ratestim_PIPsyn) * PI_PM)
(k_PIP2hyd * (PIP2_PM * PLC_act_PM))
((kBasalSynPIP2 * 0.581 * ( - 1.0 + exp(((PIP2_basal - PIP2_PM) / PIP2_basal)))) * (PIP2_PM < PIP2_basal))
((Rate_PIP2Synbasal + Rate_PIP2SynStim) * PIP_PM)
SurfToVol_NM
(kf_IP3PH * KdIP3PH)
(((kf_IP3PH * IP3_Cyt) * PH_GFP_Cyt) - (kr_IP3PH * IP3_PHGFP_Cyt))
(SurfToVol_NM * VolFract_Nucleus / (1.0 - VolFract_Nucleus))
(SurfToVol_PM / (1.0 - VolFract_Nucleus))
0.0
(kIP3deg * (IP3_Cyt - IP3_basal))
(SurfToVol_PM * VolFract_Cytosol / (1.0 - VolFract_Cytosol))
(k_PIP2PHhyd * PLC_act_PM * PIP2_PHGFP_PM)
J_IP3_PHGFP
IP3_PHGFP_Cyt_init
- Rate_PIPSyn
PI_PM_init
( - Rate_PIP2_hyd - J_PIP2_PH + Rate_PIP2Syn)
PIP2_PM_init
( - Rate_PIP2Syn + Rate_PIPSyn)
PIP_PM_init
(Rate_PIP2_hyd + RatePIP2_PH_hyd)
DAG_PM_init
RatePLCact
PLC_act_PM_init
0.05
96480.0
0.05
1.0
4000.0
10.0
0.16
0.16
2.30769
1000.0
0.1
0.1
1.0
1.0
0.0
0.12
2000.0
0.0
0.1
5.0E-4
0.0
0.08
2857.0
0.0
300.0
3.6923
100.0
0.0
1.0
(1.0 / 602.0)
2857.0
0.8
1.0
2.0
4000.0
0.5
0.05
142857.0
0.019
0.0055
0.0
1.0
0.048
0.16
4617.4
2.4
8314.0
0.92
0.05
96480.0
0.05
1.0
4000.0
10.0
0.16
0.16
(2.30769 * 3.0)
1000.0
0.1
0.1
1.0
1.0
0.0
0.12
2000.0
0.0
0.1
5.0E-4
0.0
0.08
2857.0
0.0
300.0
(3.6923 * 3.0)
100.0
0.0
1.0
(1.0 / 602.0)
2857.0
0.8
1.0
2.0
4000.0
0.5
0.05
142857.0
0.019
0.0055
0.0
1.0
0.048
0.16
(4617.4 * 3.0)
2.4
8314.0
0.92
0.05
96480.0
0.05
1.0
4000.0
10.0
0.16
0.16
(2.30769 * 0.3)
1000.0
0.1
0.1
1.0
1.0
0.0
0.12
2000.0
0.0
0.1
5.0E-4
0.0
0.08
2857.0
0.0
300.0
(3.6923 * 0.3)
100.0
0.0
1.0
(1.0 / 602.0)
2857.0
0.8
1.0
2.0
4000.0
0.5
0.05
142857.0
0.019
0.0055
0.0
1.0
0.048
0.16
(4617.4 * 0.3)
2.4
8314.0
0.92
0.05
96480.0
0.05
1.0
4000.0
10.0
0.16
0.16
2.30769
1000.0
0.1
0.1
1.0
1.0
0.0
0.12
2000.0
0.0
0.1
5.0E-4
0.0
0.08
2857.0
0.0
300.0
3.6923
100.0
0.0
1.0
(1.0 / 602.0)
2857.0
0.8
1.0
0.1
4000.0
0.5
0.05
142857.0
0.019
0.0055
0.0
1.0
0.048
0.16
92307.6
2.4
8314.0
0.92
(copied from Compartmental Sim) (copied from uncaging) (copied from Average Exp Params - Comp) (copied from SS for Exp params) (copied from steady state compartment)
2.30769
0.0
3.6923
0.0
0.0
0.0
0.0
0.0
0.16
0.0
4617.4
0.0
142857.0
0.0
1.0
0.0
4000.0
0.0
2857.0
0.0
2000.0
0.0
100.0
0.0
0.0
0.0
2.0
0.0015
100.0
0.16
1000.0
0.0
96480.0
10.0
8314.0
1.0
2857.0
10.0
3.6923
0.0
0.01
2000.0
142857.0
1.188
2.30769
0.0
0.5
0.16
0.16
2857.0
4000.0
0.1
2.0
6.0
200.0
0.0
0.8
0.0
0.01
(1.0 / 602.0)
10.0
0.05
1.0
0.05
1.0
4617.4
1072.0
300.0
100.0
0.045
4000.0
1000.0
50.0
0.05
((PIP2_PHGFP_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PH_GFP_Cyt_init * (1.0 - VolFract_Nucleus) * VolFract_Cytosol) + (IP3_PHGFP_Cyt_init * (1.0 - VolFract_Nucleus) * VolFract_Cytosol))
(( - (PIP2_PHGFP_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE) + K_PH_GFP_Cyt_total - (IP3_PHGFP_Cyt * (1.0 - VolFract_Nucleus) * VolFract_Cytosol)) / ((1.0 - VolFract_Nucleus) * VolFract_Cytosol))
(kf_PIP2PH * KdPIP2PH)
(((kf_PIP2PH * PH_GFP_Cyt) * PIP2_PM) - (kr_PIP2PH * PIP2_PHGFP_PM))
((t > tau0) * exp( - ((t - tau0) / stimdecay)))
(kStimSynPIP2 * exp( - ((t - tauPIP2syn) / PIP2syndecay)) * (t > tauPIP2syn))
((PLC_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE) + (PLC_act_PM_init * SurfToVol_PM * VolFract_Cytosol * KMOLE))
((K_PLC_PM_total - (PLC_act_PM * SurfToVol_PM * VolFract_Cytosol * KMOLE)) / (SurfToVol_PM * VolFract_Cytosol * KMOLE))
((KfPLCact * (PLC_PM * stim_PM * signal)) - (krPLCact * PLC_act_PM))
((kBasalSynPIP * 0.581 * ( - 1.0 + exp(((PIP_basal - PIP_PM) / PIP_basal)))) * (PIP_PM < PIP_basal))
(kStimSynPIP * exp( - ((t - tauPIPsyn) / PIPsyndecay)) * (t > tauPIPsyn))
((Ratebasal_PIPsyn + Ratestim_PIPsyn) * PI_PM)
(k_PIP2hyd * (PIP2_PM * PLC_act_PM))
((kBasalSynPIP2 * 0.581 * ( - 1.0 + exp(((PIP2_basal - PIP2_PM) / PIP2_basal)))) * (PIP2_PM < PIP2_basal))
((Rate_PIP2Synbasal + Rate_PIP2SynStim) * PIP_PM)
SurfToVol_NM
(kf_IP3PH * KdIP3PH)
(((kf_IP3PH * IP3_Cyt) * PH_GFP_Cyt) - (kr_IP3PH * IP3_PHGFP_Cyt))
(SurfToVol_NM * VolFract_Nucleus / (1.0 - VolFract_Nucleus))
(SurfToVol_PM / (1.0 - VolFract_Nucleus))
0.0
(kIP3deg * (IP3_Cyt - IP3_basal))
(SurfToVol_PM * VolFract_Cytosol / (1.0 - VolFract_Cytosol))
(k_PIP2PHhyd * PLC_act_PM * PIP2_PHGFP_PM)
J_IP3_PHGFP
IP3_PHGFP_Cyt_init
((RatePIP2_PH_hyd * Kflux_PM_Cytosol * KMOLE) + (Rate_PIP2_hyd * Kflux_PM_Cytosol * KMOLE) - Rate_IP3deg - J_IP3_PHGFP)
IP3_Cyt_init
(J_PIP2_PH - RatePIP2_PH_hyd)
PIP2_PHGFP_PM_init
- Rate_PIPSyn
PI_PM_init
( - Rate_PIP2Syn + Rate_PIPSyn)
PIP_PM_init
(Rate_PIP2_hyd + RatePIP2_PH_hyd)
DAG_PM_init
RatePLCact
PLC_act_PM_init
0.05
96480.0
0.05
1.0
4000.0
10.0
0.16
0.16
2.30769
1000.0
0.1
0.1
1.0
1.0
0.0
0.12
2000.0
0.0
0.1
5.0E-4
0.0
0.08
2857.0
0.0
300.0
3.6923
100.0
0.0
1.0
(1.0 / 602.0)
2857.0
0.8
1.0
2.0
4000.0
0.5
0.05
142857.0
0.019
0.0055
0.0
4000.0
1.0
0.048
4617.4
2.4
8314.0
0.92
0.05
96480.0
0.05
1.0
4000.0
10.0
0.16
0.16
(2.30769 * 0.3)
1000.0
0.1
0.1
1.0
1.0
0.0
0.12
2000.0
0.0
0.1
5.0E-4
0.0
0.08
2857.0
0.0
300.0
(3.6923 * 0.3)
100.0
0.0
1.0
(1.0 / 602.0)
2857.0
0.8
1.0
2.0
4000.0
0.5
0.05
142857.0
0.019
0.0055
0.0
4000.0
1.0
0.048
(4617.4 * 0.3)
2.4
8314.0
0.92
0.05
96480.0
0.05
1.0
4000.0
10.0
0.16
0.16
(2.30769 * 3.0)
1000.0
0.1
0.1
1.0
1.0
0.0
0.12
2000.0
0.0
0.1
5.0E-4
0.0
0.08
2857.0
0.0
300.0
(3.6923 * 3.0)
100.0
0.0
1.0
(1.0 / 602.0)
2857.0
0.8
1.0
2.0
4000.0
0.5
0.05
142857.0
0.019
0.0055
0.0
4000.0
1.0
0.048
(4617.4 * 3.0)
2.4
8314.0
0.92
0.05
96480.0
0.05
1.0
4000.0
10.0
0.16
0.16
2.30769
1000.0
0.1
0.1
1.0
1.0
0.0
0.12
2000.0
0.0
0.1
5.0E-4
0.0
0.08
2857.0
0.0
300.0
3.6923
100.0
0.0
1.0
(1.0 / 602.0)
2857.0
0.8
1.0
0.1
4000.0
0.5
0.05
142857.0
0.019
0.0055
0.0
4000.0
1.0
0.048
92307.6
2.4
8314.0
0.92
(copied from Comp Fixed IP3) (copied from Compartmental Sim) (copied from uncaging) (copied from Average Exp Params - Comp) (copied from SS for Exp params) (copied from steady state compartment)
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
NoName
1.76923
50.0
2.8308
50.0
0.0
0.0
0.0
0.0
0.16
283.0
3538.46
0.0
142857.0
0.0
1.0
0.0
4000.0
0.0
2857.0
0.0
2000.0
0.0
100.0
0.0
0.0
0.0
2.0
0.0015
100.0
0.16
1000.0
0.0
96480.0
10.0
8314.0
1.0
2857.0
10.0
2.8308
0.0
0.01
2000.0
142857.0
1.188
1.76923
0.0
0.16
0.16
2857.0
4000.0
2.0
6.0
200.0
0.0
0.0
0.01
(1.0 / 602.0)
10.0
0.05
0.05
1.0
3538.46
1072.0
300.0
100.0
0.045
1000.0
0.05
(kf_PIP2PH * KdPIP2PH)
(DAG_PM_init + PIP_PM_init + PIP2_PM_init + PIP2_PHGFP_PM_init + PI_PM_init)
( - DAG_PM - PIP_PM - PIP2_PM + K_PIP2_PHGFP_PM_total - PI_PM)
(((kf_PIP2PH * PH_GFP_INSIDE) * PIP2_PM) - (kr_PIP2PH * PIP2_PHGFP_PM))
((t > tau0) * exp( - ((t - tau0) / stimdecay)))
(kStimSynPIP2 * exp( - ((t - tauPIP2syn) / PIP2syndecay)) * (t > tauPIP2syn))
(PLC_PM_init + PLC_act_PM_init)
(K_PLC_PM_total - PLC_act_PM)
((KfPLCact * (PLC_PM * stim_PM * signal)) - (krPLCact * PLC_act_PM))
((kBasalSynPIP * 0.581 * ( - 1.0 + exp(((PIP_basal - PIP_PM) / PIP_basal)))) * (PIP_PM < PIP_basal))
(kStimSynPIP * exp( - ((t - tauPIPsyn) / PIPsyndecay)) * (t > tauPIPsyn))
((Ratebasal_PIPsyn + Ratestim_PIPsyn) * PI_PM)
(k_PIP2hyd * (PIP2_PM * PLC_act_PM))
((kBasalSynPIP2 * 0.581 * ( - 1.0 + exp(((PIP2_basal - PIP2_PM) / PIP2_basal)))) * (PIP2_PM < PIP2_basal))
((Rate_PIP2Synbasal + Rate_PIP2SynStim) * PIP_PM)
(kf_IP3PH * KdIP3PH)
(((kf_IP3PH * IP3) * PH_GFP) - (kr_IP3PH * IP3_PHGFP))
0.0
(kIP3deg * (IP3 - IP3_basal))
0.0
50.0
0.0
0.0
50.0
0.0
0.0
283.0
0.0
0.0
50.0
PH_GFP_Cyt_init
0.0
50.0
IP3_PHGFP_Cyt_init
- Rate_IP3deg
283.0
IP3_Cyt_init
(PH_GFP - IP3)
(IP3_PHGFP + IP3)
- J_IP3_PHGFP
0.0
50.0
0.0
0.0
50.0
0.0
0.0
283.0
0.0
- Rate_PIPSyn
PI_PM_init
( - Rate_PIP2_hyd - J_PIP2_PH + Rate_PIP2Syn)
PIP2_PM_init
( - Rate_PIP2Syn + Rate_PIPSyn)
PIP_PM_init
Rate_PIP2_hyd
DAG_PM_init
RatePLCact
PLC_act_PM_init
(KMOLE * ( - 1.0 * J_PIP2_PH))
0.0
0.0
0.0
(KMOLE * Rate_PIP2_hyd)
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
1.0
300.0
(1.0 / 602.0)
2000.0
0.0
2.0
142857.0
96480.0
1.76923
5.0E-4
10.0
1.0
0.048
0.05
0.1
0.92
0.0
0.019
100.0
1.0
1.0
0.0055
0.05
0.05
0.0
0.12
2.8308
0.08
3538.46
0.16
8314.0
4000.0
4000.0
0.1
0.0
0.16
2.4
2857.0
0.0
2857.0
1000.0
1.0
1.0
300.0
(1.0 / 602.0)
2000.0
0.0
2.0
142857.0
96480.0
1.76923
5.0E-4
10.0
1.0
0.048
0.05
0.1
0.92
0.0
0.019
100.0
1.0
1.0
0.0055
0.05
0.05
0.0
0.12
2.8308
0.08
3538.46
0.16
8314.0
4000.0
4000.0
0.1
0.0
0.16
2.4
2857.0
0.0
2857.0
1000.0
1.0
1s test to check ss before 50ms
1.0
300.0
(1.0 / 602.0)
2000.0
0.0
2.0
142857.0
96480.0
1.76923
5.0E-4
10.0
1.0
0.048
0.05
0.1
0.92
0.0
0.019
100.0
1.0
1.0
0.0055
0.05
0.05
0.0
0.12
2.8308
0.08
3538.46
0.16
8314.0
4000.0
4000.0
0.1
0.0
0.16
2.4
2857.0
0.0
2857.0
1000.0
1.0
1s test to check if shorter timestep is required
1.0
300.0
(1.0 / 602.0)
2000.0
0.0
2.0
(142857.0 * 4.0)
96480.0
1.76923
5.0E-4
10.0
1.0
0.048
0.05
0.1
0.92
0.0
0.019
100.0
1.0
1.0
0.0055
0.05
0.05
0.0
0.12
2.8308
0.08
(3538.46 * 4.0)
0.16
8314.0
(4000.0 * 4.0)
(4000.0 * 4.0)
0.1
0.0
0.16
2.4
(2857.0 * 4.0)
0.0
(2857.0 * 4.0)
1000.0
1.0
4X membrane species except PLC, to account for 3D S:V This has the effect of increasing the rate of production of IP3 without affecting the relative rates of change of membrane species All membrane variables will be divided by 4 to correct for this in the presentation of the data
1.0
300.0
(1.0 / 602.0)
2000.0
0.0
2.0
(142857.0 * 4.0)
96480.0
0.0
5.0E-4
10.0
1.0
0.048
0.05
0.1
0.92
0.0
0.019
100.0
1.0
1.0
0.0055
0.05
0.05
0.0
0.12
0.0
0.08
0.0
0.16
8314.0
(4000.0 * 4.0)
(4000.0 * 4.0)
0.1
0.0
0.16
2.4
(2857.0 * 4.0)
0.0
(2857.0 * 4.0)
1000.0
1.0
4X membrane species to account for 3D S:V No PH-GFP
1.0
300.0
(1.0 / 602.0)
2000.0
0.0
2.0
(142857.0 * 4.0)
96480.0
0.0
5.0E-4
10.0
1.0
0.048
0.05
0.1
0.92
0.0
0.019
100.0
1.0
1.0
0.0055
0.05
0.05
0.0
0.12
0.0
0.08
0.0
0.16
8314.0
(4000.0 * 4.0)
(4000.0 * 4.0)
0.1
0.0
0.16
2.4
(2857.0 * 4.0)
0.0
(2857.0 * 4.0)
1000.0
1.0
4X membrane species to account for 3D S:V No PH-GFP Higher spatial resolution
(copied from Image-based Geom) (copied from Compartmental Sim) (copied from uncaging) (copied from Average Exp Params - Comp) (copied from SS for Exp params) (copied from steady state compartment)
This model is intended for anlaysis of PH-GFP experiments and includes binding of the probe to both PIP2 on the plasma membrane and IP3 in the cytosol. The system is based on bradykinin induced calcium release mediated by IP3 in N1E-115 neuroblastoma cells.