NRCAM, the National Resource for Cell Analysis and Modeling is developing a unique software modeling environment, the Virtual Cell, for quantitative cell biological research. NRCAM is currently funded through the NCRR, National Center for Research Resources, a component of the National Institutes of Health (NIH).

Approaches in computational cell biology are coupled with high resolution light microscopy to facilitate the interplay between experimental manipulation and computational simulation of specific cellular functions that can range from simple molecular motors to tissue-wide process. The Virtual Cell is deployed as a distributed application that is used over the Internet. It is freely accessible to all members of the scientific community.

The Virtual Cell has been specifically designed to be a tool for a wide range of scientists, from experimental cell biologists to theoretical biophysicists. Likewise the creation of models can range from the simple, to evaluate hypotheses or to interpret experimental data, to complex multi-layered models used to probe the predicted behavior of complex, highly non-linear systems. Such models can be based on both experimental data and purely theoretical assumptions.

The user can build complex models with a web-based Java interface to specify compartmental topology and geometry, molecular characteristics, and relevant interaction parameters. The Virtual Cell automatically converts the biological description into a corresponding mathematical system of ordinary and/or partial differential equations. Distinct biological and mathematical frameworks are encompassed within a single graphical interface. The mathematic-savy user may directly specify the complete mathematical description of the model, bypassing the schematic interface. The Virtual Cell will then solve the equations by applying numerical solvers and generate appropriate software code to perform and analyze simulations. Results can be displayed and analyzed on-line or downloaded to the users computer in a variety of formats.

NRCAM resides at the Center for Cell Analysis and Modeling, CCAM, at the University of Connecticut Health Center, UCHC. CCAM provides a vast array of laboratory equipment that can be used for obtaining experimental data needed to create and enhance Virtual Cell models. Microscopy instrumentation includes three confocal laser scanning microscopes including UV excitation, nonlinear optical microscopy utilizing a titanium sapphire pulsed laser, confocal-based fluorescence correlation spectroscopy, wide-field imaging workstation with cooled CCD and rapid excitation filter wheel, and dual-wavelength spectrofluorometer. Access to the facilities and technical staff is open to all researchers.